Induction of endogenous appearance of ZKSCAN3 by using CRISPR-dCas9 transcriptional activation system ZKSCAN3 +/+ and ZKSCAN3 -/ - hMSCs are obtained enzymatically by TrypLE (Gibco) and centrifuged at 1500 grams for 5 minute at RT. The pellets happened to be fixed with 4% glutaraldehyde in PBS at 4A°C instantly. Samples are dehydrated in a graded...
